A composite gene delivery system consisting of polyethylenimine and an amphipathic peptide KALA
Identifieur interne : 001D85 ( Main/Exploration ); précédent : 001D84; suivant : 001D86A composite gene delivery system consisting of polyethylenimine and an amphipathic peptide KALA
Auteurs : Sang-Hyun Min [Corée du Sud] ; Dong Chul Lee [Corée du Sud] ; Mi Jung Lim [Corée du Sud] ; Hyun Sook Park [Corée du Sud] ; Dong Min Kim [Corée du Sud] ; Cheong Weon Cho [Corée du Sud] ; Do Young Yoon [Corée du Sud] ; Young Il Yeom [Corée du Sud]Source :
- The Journal of Gene Medicine [ 1099-498X ] ; 2006-12.
English descriptors
- Teeft :
- Appropriate amount, Band intensity, Biol chem, Cationic, Cationic gene carriers, Cationic polymer, Cell surface, Cellular, Cellular entry, Cellular entry step, Chamber slide, Charge interaction, Charge ratio, Chloroquine, Commercial cationic liposome, Commercial liposome, Compact particles, Composite formulae, Composite gene delivery system, Control release, Copyright, Core part, Cytosolic nucleases, Distinct steps, Drug deliv, Endocytic vesicle formation, Endosomal pathway, Endosome, Endosome formation, Endosomolytic, Endosomolytic activity, Fresh medium, Fusogenic, Fusogenic peptide, Gene, Gene carrier, Gene delivery, Gene delivery process, Gene delivery vehicle, Gene expression, Gene promoter, Gene transfer, Horse serum, Human genomics, John wiley sons, Kala, Korea research institute, Lipid, Luciferase, Luciferase activity, Luciferase reporter plasmid, Mammalian cells, Membrane fusion, Membrane lipids, Monolithic, Myoblast cells, Nonviral, Nonviral gene delivery systems, Nuclear localization, Oligonucleotide, Oligonucleotide probe, Other cells, Outer layer, Peak transfection, Peptide, Pgl2, Pgl2 reporter, Plasma membrane, Plasmid, Polyethylenimine, Positive charge, Positive surface charge, Primer pair, Proc natl acad, Quantitative control, Reporter plasmid, Second step, Serum proteins, Single molecule, Single system, Stock solution, Successful gene delivery system, Surface charge, Taejon korea, Target cells, Time points, Transfected, Transfected cells, Transfection, Tumor tissue, Uorescence microscope, Various cell types, Weight ratio.
Abstract
Background: Animal viruses such as enveloped virus carry multi‐functional proteins in the virion that can mediate more than two distinct steps of a gene delivery process during the transfer of viral genome into host cells. We tested if the aspects of the viral gene delivery mechanism could be mimicked by forming composite formulae from multi‐functional synthetic gene carriers having complementary action modes. Methods: Polyethylenimine (PEI) was chosen as the component responsible for endosome escape and DNA condensation and KALA for cellular entry and DNA condensation. Compact DNA‐carrier particles consisting of the core part where DNA chains were tightly condensed by PEI and the outer layer lined with KALA were formulated, characterized and compared with monolithic cationic formulae in terms of gene delivery efficiency and mechanism. Results: High‐level gene expression was observed when C2C12 cells were transfected with DNA that was first partially condensed with PEI and, then, fully with KALA. In these formulae KALA mediated enhanced cellular entry of DNA by facilitating endocytic vesicle formation, while PEI provided an effective endosomolytic capacity. An optimal PEI/KALA formula showed transfection efficiencies better than or comparable to the commercial cationic liposome in various cell types in culture and in vivo. Conclusions: Gene delivery by combining the membrane‐active property of KALA with the endosomolytic activity of PEI can be more efficient than that by either of the properties alone. It appears that, in these formulae, the predominant role of KALA is to facilitate cellular entry of DNA by providing a fusogenic capability, rather than an endosomolytic activity. Copyright © 2006 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jgm.973
Affiliations:
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Cho</name></author><author><name sortKey="Yoon, Do Young" sort="Yoon, Do Young" uniqKey="Yoon D" first="Do Young" last="Yoon">Do Young Yoon</name></author><author><name sortKey="Yeom, Young Il" sort="Yeom, Young Il" uniqKey="Yeom Y" first="Young Il" last="Yeom">Young Il Yeom</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CBFCD0933B13C697F1A6B5FC4FCD9FF252159E7D</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1002/jgm.973</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-H7QFWDLC-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000965</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000965</idno><idno type="wicri:Area/Istex/Curation">000965</idno><idno type="wicri:Area/Istex/Checkpoint">000C53</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C53</idno><idno type="wicri:doubleKey">1099-498X:2006:Min 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Yusong‐gu, Taejon 305‐333</wicri:regionArea><wicri:noRegion>Taejon 305‐333</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Dong Min" sort="Kim, Dong Min" uniqKey="Kim D" first="Dong Min" last="Kim">Dong Min Kim</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun‐dong, Yusong‐gu, Taejon 305‐333</wicri:regionArea><wicri:noRegion>Taejon 305‐333</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Cheong Weon" sort="Cho, Cheong Weon" uniqKey="Cho C" first="Cheong Weon" last="Cho">Cheong Weon Cho</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Pharmaceutics, College of Pharmacy, Chungnam National University, 220 Gung‐dong, Yuseong‐gu Taejeon 305‐764</wicri:regionArea><wicri:noRegion>Yuseong‐gu Taejeon 305‐764</wicri:noRegion></affiliation></author><author><name sortKey="Yoon, Do Young" sort="Yoon, Do Young" uniqKey="Yoon D" first="Do Young" last="Yoon">Do Young Yoon</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Cell Biology, Department of Bioscience and Biotechnology, Konkuk University, Seoul 143‐701</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Yeom, Young Il" sort="Yeom, Young Il" uniqKey="Yeom Y" first="Young Il" last="Yeom">Young Il Yeom</name><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun‐dong, Yusong‐gu, Taejon 305‐333</wicri:regionArea><wicri:noRegion>Taejon 305‐333</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Correspondence address: Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun‐dong, Yusong‐gu, Taejon 305‐333</wicri:regionArea><wicri:noRegion>Taejon 305‐333</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Gene Medicine</title><title level="j" type="alt">JOURNAL OF GENE MEDICINE, THE</title><idno type="ISSN">1099-498X</idno><idno type="eISSN">1521-2254</idno><imprint><biblScope unit="vol">8</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1425">1425</biblScope><biblScope unit="page" to="1434">1434</biblScope><biblScope unit="page-count">10</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2006-12">2006-12</date></imprint><idno type="ISSN">1099-498X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-498X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Appropriate amount</term><term>Band intensity</term><term>Biol chem</term><term>Cationic</term><term>Cationic gene carriers</term><term>Cationic polymer</term><term>Cell surface</term><term>Cellular</term><term>Cellular entry</term><term>Cellular entry step</term><term>Chamber slide</term><term>Charge interaction</term><term>Charge ratio</term><term>Chloroquine</term><term>Commercial cationic liposome</term><term>Commercial liposome</term><term>Compact particles</term><term>Composite formulae</term><term>Composite gene delivery system</term><term>Control release</term><term>Copyright</term><term>Core part</term><term>Cytosolic nucleases</term><term>Distinct steps</term><term>Drug deliv</term><term>Endocytic vesicle formation</term><term>Endosomal pathway</term><term>Endosome</term><term>Endosome formation</term><term>Endosomolytic</term><term>Endosomolytic activity</term><term>Fresh medium</term><term>Fusogenic</term><term>Fusogenic peptide</term><term>Gene</term><term>Gene carrier</term><term>Gene delivery</term><term>Gene delivery process</term><term>Gene delivery vehicle</term><term>Gene expression</term><term>Gene promoter</term><term>Gene transfer</term><term>Horse serum</term><term>Human genomics</term><term>John wiley sons</term><term>Kala</term><term>Korea research institute</term><term>Lipid</term><term>Luciferase</term><term>Luciferase activity</term><term>Luciferase reporter plasmid</term><term>Mammalian cells</term><term>Membrane fusion</term><term>Membrane lipids</term><term>Monolithic</term><term>Myoblast cells</term><term>Nonviral</term><term>Nonviral gene delivery systems</term><term>Nuclear localization</term><term>Oligonucleotide</term><term>Oligonucleotide probe</term><term>Other cells</term><term>Outer layer</term><term>Peak transfection</term><term>Peptide</term><term>Pgl2</term><term>Pgl2 reporter</term><term>Plasma membrane</term><term>Plasmid</term><term>Polyethylenimine</term><term>Positive charge</term><term>Positive surface charge</term><term>Primer pair</term><term>Proc natl acad</term><term>Quantitative control</term><term>Reporter plasmid</term><term>Second step</term><term>Serum proteins</term><term>Single molecule</term><term>Single system</term><term>Stock solution</term><term>Successful gene delivery system</term><term>Surface charge</term><term>Taejon korea</term><term>Target cells</term><term>Time points</term><term>Transfected</term><term>Transfected cells</term><term>Transfection</term><term>Tumor tissue</term><term>Uorescence microscope</term><term>Various cell types</term><term>Weight ratio</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Animal viruses such as enveloped virus carry multi‐functional proteins in the virion that can mediate more than two distinct steps of a gene delivery process during the transfer of viral genome into host cells. We tested if the aspects of the viral gene delivery mechanism could be mimicked by forming composite formulae from multi‐functional synthetic gene carriers having complementary action modes. Methods: Polyethylenimine (PEI) was chosen as the component responsible for endosome escape and DNA condensation and KALA for cellular entry and DNA condensation. Compact DNA‐carrier particles consisting of the core part where DNA chains were tightly condensed by PEI and the outer layer lined with KALA were formulated, characterized and compared with monolithic cationic formulae in terms of gene delivery efficiency and mechanism. Results: High‐level gene expression was observed when C2C12 cells were transfected with DNA that was first partially condensed with PEI and, then, fully with KALA. In these formulae KALA mediated enhanced cellular entry of DNA by facilitating endocytic vesicle formation, while PEI provided an effective endosomolytic capacity. An optimal PEI/KALA formula showed transfection efficiencies better than or comparable to the commercial cationic liposome in various cell types in culture and in vivo. Conclusions: Gene delivery by combining the membrane‐active property of KALA with the endosomolytic activity of PEI can be more efficient than that by either of the properties alone. It appears that, in these formulae, the predominant role of KALA is to facilitate cellular entry of DNA by providing a fusogenic capability, rather than an endosomolytic activity. Copyright © 2006 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Min, Sang Yun" sort="Min, Sang Yun" uniqKey="Min S" first="Sang-Hyun" last="Min">Sang-Hyun Min</name></noRegion><name sortKey="Cho, Cheong Weon" sort="Cho, Cheong Weon" uniqKey="Cho C" first="Cheong Weon" last="Cho">Cheong Weon Cho</name><name sortKey="Kim, Dong Min" sort="Kim, Dong Min" uniqKey="Kim D" first="Dong Min" last="Kim">Dong Min Kim</name><name sortKey="Lee, Dong Chul" sort="Lee, Dong Chul" uniqKey="Lee D" first="Dong Chul" last="Lee">Dong Chul Lee</name><name sortKey="Lim, Mi Jung" sort="Lim, Mi Jung" uniqKey="Lim M" first="Mi Jung" last="Lim">Mi Jung Lim</name><name sortKey="Park, Hyun Sook" sort="Park, Hyun Sook" uniqKey="Park H" first="Hyun Sook" last="Park">Hyun Sook Park</name><name sortKey="Yeom, Young Il" sort="Yeom, Young Il" uniqKey="Yeom Y" first="Young Il" last="Yeom">Young Il Yeom</name><name sortKey="Yeom, Young Il" sort="Yeom, Young Il" uniqKey="Yeom Y" first="Young Il" last="Yeom">Young Il Yeom</name><name sortKey="Yeom, Young Il" sort="Yeom, Young Il" uniqKey="Yeom Y" first="Young Il" last="Yeom">Young Il Yeom</name><name sortKey="Yoon, Do Young" sort="Yoon, Do Young" uniqKey="Yoon D" first="Do Young" last="Yoon">Do Young Yoon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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